Rat SMAGP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3967
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat SMAGP
Met1-Thr34, Phe56-Ile97
Accession # Q7TPF1
Met1-Thr34, Phe56-Ile97
Accession # Q7TPF1
Specificity
Detects rat SMAGP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human SMAGP is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Rat SMAGP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat pancreas
Sample: Perfusion fixed frozen sections of rat pancreas
Western Blot
0.1 µg/mL
Sample: Recombinant Rat SMAGP
Sample: Recombinant Rat SMAGP
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMAGP
SMAGP (small transmembrane and glycosylated protein) is a 25 kDa, type III transmembrane glycoprotein that participates in epithelial cell polarity. Rat SMAGP is 97 amino acids (aa) in length and contains no signal sequence. The N-terminal 34 amino acids constitute the extracellular domain and show O-linked glycosylation. The C‑terminal 42 amino acids (56‑97) make up the cytoplasmic region. SMAGP is likely involved in cell adhesion and communication. Relocation of SMAGP from the membrane to the cytosol is associated with tissue disorganization and metastatic transformation. Over the extracellular and cytoplasmic domains, rat SMAGP shares 96% and 66% aa sequence identity with mouse and human SMAGP, respectively.
Long Name
Small Transmembrane and Glycosylated Protein
Alternate Names
LOC57228
Gene Symbol
SMAGP
UniProt
Additional SMAGP Products
Product Documents for Rat SMAGP Antibody
Product Specific Notices for Rat SMAGP Antibody
For research use only
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