RB1 Antibody (172C1094) - (cleaved)

Knockout/Knockdown, Biological Validation

Validated:

Cited:

Unconjugated

Monoclonal Mouse IgG₁ Clone # 172C1094

0.5 mg/ml

A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.

The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).

Monoclonal

Mouse

IgG₁

Protein G purified

PBS containing 0.05% BSA

0.05% Sodium Azide

0.5 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.