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RB1 Antibody (RB1/7080R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20404

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RB1/7080R

Concentration

0.2 mg/ml

Product Summary for RB1 Antibody (RB1/7080R)

Immunogen

Recombinant human full-length RB1 protein

Epitope

aa 703-772

Localization

Nucleus.

Specificity

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product Its epitope is localized between aa 703-772 It shows no cross reaction with p107 or p130 It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells It does not stain the Rb-negative BT549 cells It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for RB1 Antibody (RB1/7080R)

RB1 Antibody (RB1/7080R)

RB1 Antibody (RB1/7080R)

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with RB1 antibody (RB1/7080R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for RB1 Antibody (RB1/7080R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RB1

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Gene Symbol

RB1

Additional RB1 Products

Product Documents for RB1 Antibody (RB1/7080R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RB1 Antibody (RB1/7080R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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