RB1 Antibody (RB1/7080R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20404
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # RB1/7080R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human full-length RB1 protein
Epitope
aa 703-772
Localization
Nucleus.
Specificity
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product Its epitope is localized between aa 703-772 It shows no cross reaction with p107 or p130 It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells It does not stain the Rb-negative BT549 cells It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for RB1 Antibody (RB1/7080R)
RB1 Antibody (RB1/7080R)
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with RB1 antibody (RB1/7080R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for RB1 Antibody (RB1/7080R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RB1
Long Name
Retinoblastoma-associated protein
Alternate Names
OSRC, p105-Rb, pp110
Gene Symbol
RB1
Additional RB1 Products
Product Documents for RB1 Antibody (RB1/7080R)
Product Specific Notices for RB1 Antibody (RB1/7080R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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