RB1 [p Ser795] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82176

Novus Biologicals, part of Bio-Techne
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NB100-82176
NB100-82176-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RB1 Antibodies »

Product Summary for RB1 [p Ser795] Antibody

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human RB1 around the phosphorylation site of serine 795 (P-S-SP-P-L).

Modification

p Ser795

Specificity

Detects endogenous levels of RB1 only when phosphorylated at serine 795.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RB1 [p Ser795] Antibody

Western Blot: RB1 [p Ser795] Antibody [NB100-82176] - Analysis of RB1 [p Ser795] ( using NB100-82176 in K562 cells untreated or treated with 10% serum after 48 hours of starvation.
Immunocytochemistry/ Immunofluorescence: RB1 [p Ser795] Antibody [NB100-82176]

Immunocytochemistry/ Immunofluorescence: RB1 [p Ser795] Antibody [NB100-82176]

Immunocytochemistry/Immunofluorescence: RB1 [p Ser795] Antibody [NB100-82176] - Staining of methanol-fixed Hela cells using Rb1 (P Ser795) antibody

Immunohistochemistry-Paraffin: RB1 [p Ser795] Antibody [NB100-82176] -

Immunohistochemistry-Paraffin: RB1 [p Ser795] Antibody [NB100-82176] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-82176 (left) or the same antibody preincubated with blocking peptide (right).

Applications for RB1 [p Ser795] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RB1

Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Entrez Gene IDs

5925 (Human); 19645 (Mouse); 24708 (Rat)

Gene Symbol

RB1

UniProt

P06400

Additional RB1 Products

Product Documents for RB1 [p Ser795] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RB1 [p Ser795] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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