RBBP4/RbAp48 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-60399
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Bovine (100%), Chicken (100%), Orangutan (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human retinoblastoma binding protein 4 (retinoblastoma-binding protein p48) using the numbering given in entry CAA52321.1 (GeneID 5928).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RBBP4/RbAp48 Antibody
![Western Blot: RBBP4/RbAp48 Antibody [NB100-60399]](https://resources.bio-techne.com/images/products/RBBP4-RbAp48-Antibody-Western-Blot-NB100-60399-img0004.jpg "Western Blot: RBBP4/RbAp48 Antibody [NB100-60399]")
Western Blot: RBBP4/RbAp48 Antibody [NB100-60399]
Western Blot: RBBP4/RbAp48 Antibody [NB100-60399] - Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: NB100-60399 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate.![Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NB100-60399]](https://resources.bio-techne.com/images/products/RBBP4-RbAp48-Antibody-Immunohistochemistry-NB100-60399-img0008.jpg "Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NB100-60399]")
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NB100-60399]
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NB100-60399] - Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti- RBBP4/RbAp48 used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for RBBP4/RbAp48 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200- 1:1000
Immunohistochemistry-Paraffin
1:200- 1:1000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RBBP4
Long Name
Retinoblastoma Binding Protein 4
Alternate Names
CAF-1 p48, CAF-1C, NURF55, RBAP48
Gene Symbol
RBBP4
Additional RBBP4 Products
Product Documents for RBBP4/RbAp48 Antibody
Product Specific Notices for RBBP4/RbAp48 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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