RBBP4/RbAp48 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46858

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-46858-25ul
NBP2-46858
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBBP4 Antibodies »

Product Summary for RBBP4/RbAp48 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBBP4/RbAp48 Antibody

Western Blot: RBBP4/RbAp48 Antibody [NBP2-46858]

Western Blot: RBBP4/RbAp48 Antibody [NBP2-46858]

Western Blot: RBBP4/RbAp48 Antibody [NBP2-46858] - RbAp46 Antibody [NBP2-46858] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody [NBP2-46858]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody [NBP2-46858]

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody [NBP2-46858] - Staining of human cell line HeLa shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NBP2-46858]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NBP2-46858]

Immunohistochemistry-Paraffin: RbAp46 Antibody [NBP2-46858] - Staining of human tonsil shows strong nuclear positivity.

Applications for RBBP4/RbAp48 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP4

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RBBP4/RbAp48 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.