RBBP6 Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49535V

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-49535AF350

Alexa Fluor 405

NBP1-49535AF405

Alexa Fluor 488

NBP1-49535AF488

Alexa Fluor 532

NBP1-49535AF532

Alexa Fluor 594

NBP1-49535AF594

Alexa Fluor 647

NBP1-49535AF647

Alexa Fluor 700

NBP1-49535AF700

Alexa Fluor 750

NBP1-49535AF750

Allophycocyanin

NBP1-49535APC

Biotin

NBP1-49535B

CoraFluor 1

NBP1-49535CL1

DyLight 350

NBP1-49535UV

DyLight 488

NBP1-49535G

DyLight 550

NBP1-49535R

DyLight 594

NBP1-49535DL594

DyLight 650

NBP1-49535C

DyLight 680

NBP1-49535FR

DyLight 755

NBP1-49535IR

FITC

NBP1-49535F

HRP

NBP1-49535H

Janelia Fluor 525

NBP1-49535JF525

Janelia Fluor 549

NBP1-49535JF549

Janelia Fluor 585

NBP1-49535JF585

Janelia Fluor 635

NBP1-49535JF635

Janelia Fluor 646

NBP1-49535JF646

Janelia Fluor 669

NBP1-49535JF669

mFluor Violet 450 SE

NBP1-49535MFV450

mFluor Violet 500 SE

NBP1-49535MFV500

mFluor Violet 610 SE

NBP1-49535MFV610

PE

NBP1-49535PE

PerCP

NBP1-49535PCP

Forumulation

Catalog #

BSA Free

NBP1-49535

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RBBP6 Antibodies »

Product Summary for RBBP6 Antibody [DyLight 405]

Immunogen

A synthetic peptide made to an internal portion of the human RBBP6 protein (between residues 1600-1650) [UniProt Q7Z6E9]

Localization

Nucleus nucleolus. Chromosome. Note: Colocalizes with mitotic chromosomes.

Specificity

This antibody may detect isoform 1 (201.6 kDa), isoform 2 (197.3 kDa) and isoform 4 (106.0 kDa) of human RBBP6.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for RBBP6 Antibody [DyLight 405]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RBBP6

RBBP6 (retinoblastoma binding protein 6; P2PR, PACT, RBQ1) is a multidomain protein with an expected molecular size of about 250kD and it colocalizes with mitotic chromosomes as well as with NEK6 in centrosomes. RBBP6 is highly expressed in normal placenta and testis, and is overexpressed in certain cancers (esophageal and lung cancer). RBBP6 is a multifunctional protein which is found presumably in all eukaryotes and is implicated in versatile cellular activities such as mRNA metabolism, cell cycle regulation, carcinogenesis, and development. It forms part of the pre-mRNA 3-end processing complex in humans and the cleavage/polyadenylation factor complex in yeast. RBBP6 interacts with p53/TP53, RB1, NEK6, MDM2, YBX1 etc., and gets phosphorylated upon DNA damage via ATM/ATR or through NEK6. RBBP6 is an E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, thereby facilitating its proteasomal degradation. RBBP6 also acts as a scaffold protein to enhance the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53, thereby acting as a negative regulator of p53/TP53.

Alternate Names

E3 ubiquitin-protein ligase RBBP6, EC 6.3.2.-, MY038, P2PR, P2P-R, p53-associated cellular protein of testis, PACTDKFZp761B2423, Proliferation potential-related protein, Protein P2P-R, RB-binding Q-protein 1, RBQ1, RBQ-1, retinoblastoma binding protein 6, Retinoblastoma-binding protein 6DKFZp686P0638, Retinoblastoma-binding Q protein 1, SNAMA

Gene Symbol

RBBP6

Additional RBBP6 Products

Product Documents for RBBP6 Antibody [DyLight 405]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for RBBP6 Antibody [DyLight 405]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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