RBCK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27105

Novus Biologicals, part of Bio-Techne
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NBP2-27105
NBP2-27105SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RBCK1 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 150-200 from human RBCk1 was used as the immunogen for this antibody.

Reactivity Notes

Orangutan, Chimpanzee (100%), New world monkey (89%), Bovine, Guinea Pig, Mole Rat (83%), Canine, Elephant, Rabbit (78%), Panda, Oppossum (67%), Rat (61%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBCK1 Antibody

Western Blot: RBCK1 Antibody [NBP2-27105]

Western Blot: RBCK1 Antibody [NBP2-27105]

Western Blot: RBCK1 Antibody [NBP2-27105] - Analysis of RBCK1 using RBCK1 antibody. A549 cell lysate in the 1) pre immune sera and 2) abesence of immunizing peptide and 3) 3T3 cell lysate probed with 2 ug/ml of RBCK1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: RBCK1 Antibody [NBP2-27105]

Immunocytochemistry/ Immunofluorescence: RBCK1 Antibody [NBP2-27105]

Immunocytochemistry/Immunofluorescence: RBCK1 Antibody [NBP2-27105] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RBCK1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for RBCK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-10 ug/ml

Western Blot

2-4ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBCK1

RBCk1, also known as HOIL-1L, acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex that contains a zf-RanBP (Zn-finger in Ran binding protein) and a zf-C3HC4 (RING finger) domain. RBCk1 catalyzes the ubiquitination and degradation of IRF3. Both RBCk1 and IRF3 are activated by viral infections and IRF3 is a prerequisite trigger for induction of type I Interferons (1). RBCk1 also negatively regulates TNF and IL1 induced NF-fUB activation (2). Thus it appears RBCk1 and IRF3 interaction represents a controlling antiviral regulatory channel but also that RBCk1 and ubiquitination pathways (3, 4) also are related to NF-kB and its role as an anti-viral response pathway.

Alternate Names

C20orf18, EC 6.3.2.-, HBV associated factor 4, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, HOIL-1, RanBP-type and C3HC4-type zinc finger containing 1, ranBP-type and C3HC4-type zinc finger-containing protein 1, RBCC protein interacting with PKC1, RBCK2, RNF54RING finger protein 54, UBCE7IP3HOIL1, ubiquitin conjugating enzyme 7 interacting protein 3, Ubiquitin-conjugating enzyme 7-interacting protein 3, XAP3, XAP4chromosome 20 open reading frame 18, ZRANB4

Gene Symbol

RBCK1

UniProt

Q9BYM8 (Human)

Additional RBCK1 Products

Product Documents for RBCK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RBCK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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