RBCK1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27105
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Primate
Predicted:
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 150-200 from human RBCk1 was used as the immunogen for this antibody.
Reactivity Notes
Orangutan, Chimpanzee (100%), New world monkey (89%), Bovine, Guinea Pig, Mole Rat (83%), Canine, Elephant, Rabbit (78%), Panda, Oppossum (67%), Rat (61%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RBCK1 Antibody - BSA Free
![Western Blot: RBCK1 Antibody [NBP2-27105]](https://resources.bio-techne.com/images/products/RBCK1-Antibody-Western-Blot-NBP2-27105-img0004.jpg "Western Blot: RBCK1 Antibody [NBP2-27105]")
Western Blot: RBCK1 Antibody [NBP2-27105]
Western Blot: RBCK1 Antibody [NBP2-27105] - Analysis of RBCK1 using RBCK1 antibody. A549 cell lysate in the 1) pre immune sera and 2) abesence of immunizing peptide and 3) 3T3 cell lysate probed with 2 ug/ml of RBCK1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.![Immunocytochemistry/ Immunofluorescence: RBCK1 Antibody [NBP2-27105]](https://resources.bio-techne.com/images/products/RBCK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27105-img0005.jpg "Immunocytochemistry/ Immunofluorescence: RBCK1 Antibody [NBP2-27105]")
Immunocytochemistry/ Immunofluorescence: RBCK1 Antibody [NBP2-27105]
Immunocytochemistry/Immunofluorescence: RBCK1 Antibody [NBP2-27105] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RBCK1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Applications for RBCK1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5-10 ug/ml
Western Blot
2-4ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RBCK1
Alternate Names
C20orf18, EC 6.3.2.-, HBV associated factor 4, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, HOIL-1, RanBP-type and C3HC4-type zinc finger containing 1, ranBP-type and C3HC4-type zinc finger-containing protein 1, RBCC protein interacting with PKC1, RBCK2, RNF54RING finger protein 54, UBCE7IP3HOIL1, ubiquitin conjugating enzyme 7 interacting protein 3, Ubiquitin-conjugating enzyme 7-interacting protein 3, XAP3, XAP4chromosome 20 open reading frame 18, ZRANB4
Gene Symbol
RBCK1
UniProt
Additional RBCK1 Products
Product Documents for RBCK1 Antibody - BSA Free
Product Specific Notices for RBCK1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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