RBFOX3/NeuN Antibody (NeuN/6694R) [Janelia Fluor® 525]

Janelia Fluor 525

Recombinant Monoclonal Rabbit IgG Kappa Clone # NeuN/6694R

Please see the vial label for concentration. If unlisted please contact technical services.

A synthetic peptide corresponding to residues within aa30-60 of human RBFOX3/NeuN protein (exact sequence is proprietary) (Uniprot: A6NFN3)

Nucleus, Cytoplasm

NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Neuronal Marker

Monoclonal

Rabbit

IgG Kappa

Protein A or G purified

50mM Sodium Borate

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C in the dark.