Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RBM14 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 550 and 600 of human CoActivator Activator (RNA Binding Motif 14) using the numbering given in entry NP_006319.1 (GeneID 10432 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM14 Antibody

Western Blot: RBM14 Antibody [NB100-2884]

Western Blot: RBM14 Antibody [NB100-2884]

Western Blot: RBM14 Antibody [NB100-2884] - Detection of Human and Mouse CoAA by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), Jurkat (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-CoAA antibody NB100-2884 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: RBM14 Antibody [NB100-2884]

Immunoprecipitation: RBM14 Antibody [NB100-2884]

Immunoprecipitation: RBM14 Antibody [NB100-2884] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CoAA antibody used for IP at 6 ug/mg lysate. CoAA was also Immunoprecipitated by a previous lot this antibody. For blotting immunoprecipitated CoAA, NB100-2884 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for RBM14 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RBM14

CoAA is a RNA-binding protein. These proteins contribute to gene expression by regulating the form, abundance and stability of both coding and non-coding RNAs. In vertebrates, RNA binding proteins can also influence many processes involved in RNA processing, for example, activity-dependent transcript localization and localized protein synthesis amongst others.

Alternate Names

COAAMGC31756, Paraspeckle protein 2, PSP2DKFZp779J0927, RNA binding motif protein 14, RNA-binding motif protein 14, RNA-binding protein 14, RRM-containing coactivator activator/modulator, SIPMGC15912, Synaptotagmin-interacting protein, SYT-interacting protein, SYTIP1, TMEM137, transmembrane protein 137

Entrez Gene IDs

10432 (Human)

Gene Symbol

RBM14

UniProt

Q96PK6 (Human)

Additional RBM14 Products

Product Documents for RBM14 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RBM14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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