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RBM23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83857

Novus Biologicals, part of Bio-Techne
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NBP1-83857
NBP1-83857-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for RBM23 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MASDDFDIVIEAMLEAPYKKEEDEQQRKEVKKDYPSNTTSSTSNSGNETSGSSTIGETSNRSRDRDRYRRRNSRSRSPGRQCRHRSRSWDRRHGSESRSRDHRREDRVHYRSPPLATGYRYGHSKSPHFREKSP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM23 Antibody

Western Blot: RBM23 Antibody [NBP1-83857]

Western Blot: RBM23 Antibody [NBP1-83857]

Western Blot: RBM23 Antibody [NBP1-83857] - Analysis in control (vector only transfected HEK293T lysate) and RBM23 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RBM23 Antibody [NBP1-83857]

Immunocytochemistry/ Immunofluorescence: RBM23 Antibody [NBP1-83857]

Immunocytochemistry/Immunofluorescence: RBM23 Antibody [NBP1-83857] - Staining of human cell line U-2 OS shows localization to intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RBM23 Antibody [NBP1-83857]

Immunohistochemistry-Paraffin: RBM23 Antibody [NBP1-83857]

Immunohistochemistry-Paraffin: RBM23 Antibody [NBP1-83857] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Applications for RBM23 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 -1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM23

RBM23 encodes a member of the U2AF-like family of RNA binding proteins. This protein interacts with some steroid nuclear receptors, localizes to the promoter of a steroid- responsive gene, and increases transcription of steroid-responsive transcriptional reporters in a hormone-dependent manner. It is also implicated in the steroid receptor-dependent regulation of alternative splicing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CAPERbeta, FLJ10482, MGC4458, probable RNA-binding protein 23, RNA binding motif protein 23, RNA-binding motif protein 23, RNA-binding region (RNP1, RRM) containing 4, RNA-binding region-containing protein 4, RNPC4, Splicing factor SF2

Gene Symbol

RBM23

Additional RBM23 Products

Product Documents for RBM23 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBM23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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