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RBX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20113

Novus Biologicals, part of Bio-Techne
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NBP2-20113

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RBX1. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBX1 Antibody

Western Blot: RBX1 Antibody [NBP2-20113]

Western Blot: RBX1 Antibody [NBP2-20113]

Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
RBX1 Antibody

Western Blot: RBX1 Antibody [NBP2-20113] -

Western Blot: RBX1 Antibody [NBP2-20113] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (NBP2-20113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RBX1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RBX1

This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over. [provided by RefSeq]

Long Name

Ring-Box 1

Alternate Names

Ring Box 1, RNF75, ZYP Protein

Gene Symbol

RBX1

UniProt

Additional RBX1 Products

Product Documents for RBX1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.