RCAN1 Antibody (JE62-71)

Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100]

Western Blot: RCAN1 Antibody (JE62-71) [NBP3-32100] - Western blot analysis of RCAN1 on different lysates with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/1,000 dilution.

Lane 1: SW480 cell lysate (20 ug/Lane)
Lane 2: U-87 MG cell lysate (20 ug/Lane)
Lane 3: SH-SY5Y cell lysate (20 ug/Lane)
Lane 4: Human brain tissue lysate (40 ug/Lane)
Lane 5: Rat brain tissue lysate (40 ug/Lane)
Lane 6: Mouse brain tissue lysate (40 ug/Lane)
Lane 7: Mouse thymus tissue lysate (40 ug/Lane)
Lane 8: Rat cerebellum tissue lysate (40 ug/Lane)

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32100) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100]

Immunocytochemistry/ Immunofluorescence: RCAN1 Antibody (JE62-71) [NBP3-32100] - Immunocytochemistry analysis of Jurkat cells labeling RCAN1 with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RCAN1 antibody (NBP3-32100) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100]

Flow Cytometry: RCAN1 Antibody (JE62-71) [NBP3-32100] - Flow cytometric analysis of Jurkat cells labeling RCAN1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32100, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).