RCAN1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46853

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Rabbit

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RCAN1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of human RCAN1 (within residues 210-252). [Swiss-Prot# P53805]

Reactivity Notes

Immunogen has 92% identity to rat.

Localization

Cytoplasm, nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RCAN1 Antibody - BSA Free

Western Blot: RCAN1 AntibodyBSA Free [NBP1-46853]

Western Blot: RCAN1 AntibodyBSA Free [NBP1-46853]

Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain
Immunohistochemistry: RCAN1 Antibody - BSA Free [NBP1-46853]

Immunohistochemistry: RCAN1 Antibody - BSA Free [NBP1-46853]

Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain

Applications for RCAN1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 34272844)

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

2 ug/ml
Application Notes
In WB a band is seen at ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RCAN1

RCAN1 (regulator of calcineurin 1) inhibits the calcineurin-dependent transcriptional responses by binding to catalytic domain of calcineurin 1, a protein phosphatase involved in muscle cell differentiation/growth, T cell activation and new memory generation. Unphosphorylated RCAN1 binds/inhibits calcineurin, while RCAN1 phosphorylation at conserved serine residues interefere with this binding, resulting in increased calcineurin activity. RCAN1 over-expression mediated reduction in calcineurin activity alters the phosphorylation/activity of several calcineurin targets such as Munc18, dynamin 1, amphiphysin 1/2 and synaptojanin which are involved in exocytosis and vesicle recycling. Chronic alterations in calcineurin lead to transcriptional effects via calcineurin/NFAT signalling that regulates pre-synaptic neurotransmitter release. RCAN1 also affect exocytosis and quantal release kinetics via direct calcineurin independent mechanisms. RCAN1 is widely expressed in normal tissues/cell types with highest expression in neurons, and RCAN1 levels elevates in Down syndrome fetuses as well as Alzheimer patients, whereas, in Huntington disease RCAN1 - expression is found reduced. RCAN1 has been implicated in a variety of cellular processes, including oxidative stress, angiogenesis, mitochondrial function, immune responses and inflammation.

Alternate Names

Adapt78, calcipressin-1, calcium and oxidant-inducible mRNA, Down syndrome candidate region 1, Down syndrome critical region gene 1, Down syndrome critical region protein 1, DSC1, modulatory calcineurin-interacting protein 1, Myocyte-enriched calcineurin-interacting protein 1, near DSCR proline-rich protein, regulator of calcineurin 1CSP1DSCR1MCIP1RCN1

Entrez Gene IDs

1827 (Human); 54720 (Mouse)

Gene Symbol

RCAN1

UniProt

P53805 (Human), Q9JHG6 (Mouse)

Additional RCAN1 Products

Product Documents for RCAN1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RCAN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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