RCC1 Antibody (1C1)

Novus Biologicals, part of Bio-Techne | Catalog # H00001104-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1C1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

RCC1 (AAH07300, 312 a.a. ~ 421 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RCC1 Antibody (1C1)

Western Blot: RCC1 Antibody (1C1) [H00001104-M02]

Western Blot: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of RCC1 expression in transfected 293T cell line by RCC1 monoclonal antibody (M02), clone 1C1.Lane 1: RCC1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: RCC1 Antibody (1C1) [H00001104-M02]

Immunocytochemistry/Immunofluorescence: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of monoclonal antibody to RCC1 on HeLa cell . Antibody concentration 15 ug/ml.

Immunoprecipitation: RCC1 Antibody (1C1) [H00001104-M02]

Immunoprecipitation: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of RCC1 transfected lysate using anti-RCC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RCC1 MaxPab rabbit polyclonal antibody.

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Applications for RCC1 Antibody (1C1)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RCC1

RCC1 (regulator of chromosome condensation) is a 45 kD nuclear protein containing RCC1 repeats.This widely expressed protein associates with chromosomes and acts as a guanine nucleotide exchange factor (GEF) protein for Ras-related GTPase Ran. RCC1 is thought to maintain the Ran GTP gradient required for nucleocytoplasmic transport and spindle assembly across the interphase nuclear envelope and on condensed mitotic chromosomes. RCC1 regulates cell cycle and also plays a role in nuclear pore transport. Defects in RCC1 results in abnormal chromosome segregation and genomic instability. RCC1 is transcriptionally activated by Myc through E-box elements. RCC1 binds to chromatin in a binary complex with Ran. The Poly6164 antibody recognizes the N-terminal region of the human and mouse RCC1 protein and has been shown to be useful for Western blotting.

Alternate Names

Cell cycle regulatory protein, CHC1RCC1-I, chromosome condensation 1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, regulator of chromosome condensation, regulator of chromosome condensation 1, SNHG3-RCC1, SNHG3-RCC1 readthrough transcript

Gene Symbol

RCC1

UniProt

AAH07300.1 (Human)

Additional RCC1 Products

Product Documents for RCC1 Antibody (1C1)

Product Datasheets

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COA

SDS

Product Specific Notices for RCC1 Antibody (1C1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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