RCC1 Antibody (1F0C5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15295

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15295-100ul
NBP3-15295-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F0C5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RCC1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RCC1 (P18754). MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RCC1 Antibody (1F0C5)

Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]

Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]

Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295]

Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295]

Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295] - Human placenta using RCC1 Rabbit mAb (NBP3-15295) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]

Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]

Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Applications for RCC1 Antibody (1F0C5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RCC1

Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensationin the S phase. Binds to the chromatin. RCC1/Ran complex (together with other proteins) acts as a component of asignal transmission pathway that detects unreplicated DNA

Alternate Names

Cell cycle regulatory protein, CHC1RCC1-I, chromosome condensation 1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, regulator of chromosome condensation, regulator of chromosome condensation 1, SNHG3-RCC1, SNHG3-RCC1 readthrough transcript

Gene Symbol

RCC1

Additional RCC1 Products

Product Documents for RCC1 Antibody (1F0C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RCC1 Antibody (1F0C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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