RCOR1/CoREST Antibody (JE35-15)

Western Blot: RCOR1/CoREST Antibody (JE35-15) [NBP3-32210]

Western Blot: RCOR1/CoREST Antibody (JE35-15) [NBP3-32210] - Western blot analysis of RCOR1/CoREST on different lysates with Rabbit anti-RCOR1/CoREST antibody (NBP3-32210) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: U-2 OS cell lysate
Lane 8: HT-29 cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 65 kDa
Observed band size: 65/26 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32210) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RCOR1/CoREST Antibody (JE35-15) [NBP3-32210]

Immunohistochemistry: RCOR1/CoREST Antibody (JE35-15) [NBP3-32210] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-RCOR1/CoREST antibody (NBP3-32210) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.