RECQ1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87803

Novus Biologicals, part of Bio-Techne
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NBP1-87803
NBP1-87803-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RECQ1 Antibodies »

Product Summary for RECQ1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HVLTDAQKILCIEKCFTFTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RECQ1 Antibody

Immunocytochemistry/ Immunofluorescence: RECQ1 Antibody [NBP1-87803]

Immunocytochemistry/ Immunofluorescence: RECQ1 Antibody [NBP1-87803]

Immunocytochemistry/Immunofluorescence: RECQ1 Antibody [NBP1-87803] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803]

Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803]

Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803]

Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803]

Immunohistochemistry-Paraffin: RECQ1 Antibody [NBP1-87803] - Staining of human lymph node shows high expression.
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Applications for RECQ1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RECQ1

RECQ1 is encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described.

Alternate Names

DNA helicase, RecQ-like type 1, DNA-dependent ATPase Q1, EC 3.6.1, EC 3.6.4.12, RecQ protein-like (DNA helicase Q1-like), RecQ protein-like 1, RECQ1, RecQ1ATP-dependent DNA helicase Q1, RecQL1

Gene Symbol

RECQL

Additional RECQ1 Products

Product Documents for RECQ1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RECQ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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