Reelin Antibody (G10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1081
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # G10
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant fusion protein, corresponding to amino acids 164-496 of Mouse Reelin (derived from E15-E17 embryonic brain).
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Secreted
Specificity
Reelin (G10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
388 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Reelin Antibody (G10) - BSA Free
Immunohistochemistry-Frozen: Reelin Antibody (G10) - BSA Free [NB600-1081]
Immunohistochemistry-Frozen: Reelin Antibody (G10) [NB600-1081] - Frozen mouse brain coronal section stained with Reelin Antibody (G10). IHC-Fr image submitted by a verified customer review.Immunohistochemistry: Reelin Antibody (G10) - BSA Free [NB600-1081]
Immunohistochemistry: Reelin Antibody (G10) [NB600-1081] - Mouse anti-Reelin staining of embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone, positive for reelin using NB600-1081.Immunohistochemistry: Reelin Antibody (G10) - BSA Free [NB600-1081]
Immunohistochemistry: Reelin Antibody (G10) [NB600-1081] - Mouse anti-Reelin. Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.Applications for Reelin Antibody (G10) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 27915032).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB600-1081 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
20mM Phosphate and 0.25M NaCl
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Reelin
Alternate Names
Reeler, RELN, RL
Gene Symbol
RELN
UniProt
Additional Reelin Products
Product Documents for Reelin Antibody (G10) - BSA Free
Product Specific Notices for Reelin Antibody (G10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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