RelA/NFkB p65 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2176

Novus Biologicals, part of Bio-Techne
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NB100-2176
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the C-terminus of human NFkB p65.

Localization

Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B).

Specificity

Reacts with NFkB p65

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RelA/NFkB p65 Antibody

Western Blot: RelA/NFkB p65 Antibody [NB100-2176]

Western Blot: RelA/NFkB p65 Antibody [NB100-2176]

Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Human glomerular endothelial cell lysates, time-response treatment with 10ng/ml TNF-alpha. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176]

Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176]

Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176] - Staining of formalin-fixed, paraffin-embedded human DLBCL showing nuclear expression of NFkB p65 in the tumor cells.
Western Blot: RelA/NFkB p65 Antibody [NB100-2176]

Western Blot: RelA/NFkB p65 Antibody [NB100-2176]

Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Analysis of NFkB p65 expression in A431 (A) and HeLa (B) whole cell lysates.
View 2 more images

Applications for RelA/NFkB p65 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:400-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 27119753). Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30411846).

Reviewed Applications

Read 3 reviews rated 5 using NB100-2176 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.2% gelatin.

Preservative

0.1% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RelA/NFkB p65

p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Entrez Gene IDs

5970 (Human); 18033 (Mouse); 81736 (Rat)

Gene Symbol

RELA

UniProt

Q04206

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RelA/NFkB p65 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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