Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human reticuloendotheliosis viral oncogene homolog A using the numbering given in entry NP_068810.2 (GeneID 5970).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-224, NB100-376, NB100-464, NB500-160, NB100-381, NB200-347, NB100-2241, NBP1-06527

Scientific Data Images for RelA/NFkB p65 Antibody - BSA Free

Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831]

Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831]

Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831] - Detection of Human RelA/p65 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RelA/p65 antibody NB100-97831 used for IP at 3 mcg/mg lysate. RelA/p65 was also immunoprecipitated by rabbit anti-RelA/p65 antibody NB100-97832, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RelA/NFkB p65 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Not recommended for western blot.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RelA/NFkB p65

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Entrez Gene IDs

5970 (Human)

Gene Symbol

RELA

UniProt

Q04206

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RelA/NFkB p65 Antibody - BSA Free

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-224, NB100-376, NB100-464, NB500-160, NB100-381, NB200-347, NB100-2241, NBP1-06527

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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