Renin Antibody (009)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89723

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89723-100ul
NBP2-89723-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 009

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Renin Antibodies »

Product Summary for Renin Antibody (009)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Renin (Accession#: NP_000528.1; Met 1-Arg 406).

Specificity

Has cross-reactivity in ELISA with: Mouse REN1 / Renin-1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Renin Antibody (009)

Immunocytochemistry/ Immunofluorescence: Renin Antibody (009) [NBP2-89723]

Immunocytochemistry/ Immunofluorescence: Renin Antibody (009) [NBP2-89723]

Immunocytochemistry/Immunofluorescence: Renin Antibody (009) [NBP2-89723] - Staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: Renin Antibody (009) [NBP2-89723]

Immunohistochemistry-Paraffin: Renin Antibody (009) [NBP2-89723]

Immunohistochemistry-Paraffin: Renin Antibody (009) [NBP2-89723] - Staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution.
Flow Cytometry: Renin Antibody (009) [NBP2-89723]

Flow Cytometry: Renin Antibody (009) [NBP2-89723]

Flow Cytometry: Renin Antibody (009) [NBP2-89723] - Analysis of Human Renin expression on Jurkat cells. The cells were treated , stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for Renin Antibody (009)

Application
Recommended Usage

ELISA

1:25000-1:50000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Renin

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia.

Alternate Names

Angiotensinogenase, REN

Gene Symbol

REN

Additional Renin Products

Product Documents for Renin Antibody (009)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Renin Antibody (009)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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