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Key Product Details

Species Reactivity

Virus

Applications

ELISA, Functional, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 11-2-F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C

Specificity

This antibody reacts with the RS virus fusion glycoprotein F (also named VP70 or F0 protein), specifically to a subunit of the F protein, F1 (also named VGP48 or 48kDa), from both subgroups A and B of RS virus.This antibody has a high neutralising activity; 90% plaque neutralization titre of 102.2 (Gimenez et al 1996). This property makes it potentially useful for the preparation of reagents for prevention and/or therapeutic use (for example, vaccines or new-generation of man-made antibodies). The characterisation of the epitope of this antibody which induce high neutralising activity will be useful for the preparation of reagents (for example vaccine) to prevent RS virus infection. A RS virus vaccine is not yet available. A vaccine could be used to increase the maternal immunity to RS virus, which would then be passed to the newborn baby to provide passive immunity.The further characterisation of the epitope recognised by this antibody, which possesses a high neutralising activity, will be useful for the preparation of reagents to prevent RS virus infection. New-generation man-made antibodies, based on the 11-2-F3 antibody, could complement the effectiveness of polivizumab (Synagis), which is used to prevent but not treat established RS virus infections (Nature Biotechnology, 2013, 31, 952).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Respiratory Syncytial Virus Glycoprotein F

Alternate Names

Fusion glycoprotein F0, Fusion glycoprotein F1, Fusion glycoprotein F2

Additional Respiratory Syncytial Virus Glycoprotein F Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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