Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # RET/2795

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Ret Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 702-848) of human Ret protein (exact sequence is proprietary) (Uniprot: P07949)

Localization

Cytoplasmic, Cell Surface, Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79762).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ret Antibody (RET/2795) - Azide and BSA Free

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897]

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897]

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).
Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897]

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897]

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).
Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897]

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897]

Immunohistochemistry-Paraffin: Ret Antibody (RET/2795) - Azide and BSA Free [NBP2-79897] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with RET Mouse Monoclonal Antibody (RET/2795).
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Applications for Ret Antibody (RET/2795) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Ret

Ret, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Gene Symbol

RET

Additional Ret Products

Product Documents for Ret Antibody (RET/2795) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Ret Antibody (RET/2795) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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