Rev-erb A alpha/NR1D1 Antibody (4F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00009572-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rev-erb A alpha/NR1D1 Antibodies »

Product Specifications

Immunogen

NR1D1 (AAH56148, 1 a.a. ~ 614 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ

Specificity

NR1D1 (4F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rev-erb A alpha/NR1D1 Antibody (4F6)

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Western Blot: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - NR1D1 monoclonal antibody (M02), clone 4F6. Analysis of NR1D1 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Analysis of monoclonal antibody to NR1D1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02]

Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody (4F6) [H00009572-M02] - Representative tissue microarray specimens stained for NR1D1. Immunoreactivity of representative tumor specimens is shown. Low and high NR1D1 expression groups are defined in methods. The low NR1D1 expression ranges from immunoreactive scores 2 to 6 (left (P2xI1), middle (P3xI1), and right (P3xI2)), and the high NR1D1 expression shows immunoreactive score 12 (all, P4xI3). Image collected and cropped by CiteAb from the following publication (https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-019-1197-x) licensed under a CC-BY license.
View 11 more images

Applications for Rev-erb A alpha/NR1D1 Antibody (4F6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Reviewed Applications

Read 4 reviews rated 4.3 using H00009572-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rev-erb A alpha/NR1D1

NR1D1 functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine

Alternate Names

Ear-1, NR1D1, Reverb A alpha, THRA1, THRAL

Entrez Gene IDs

9572 (Human)

Gene Symbol

NR1D1

OMIM

602408 (Human)

UniProt

AAH56148 (Human)

Additional Rev-erb A alpha/NR1D1 Products

Product Documents for Rev-erb A alpha/NR1D1 Antibody (4F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rev-erb A alpha/NR1D1 Antibody (4F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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