Rev-erb A alpha/NR1D1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19574
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (91%), Rat (93%), Sheep (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Rev-erb A alpha/NR1D1. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rev-erb A alpha/NR1D1 Antibody
Western Blot: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] -
Western Blot: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rev-erb A alpha/NR1D1 antibody (NBP2-19574) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574]
Immunocytochemistry/Immunofluorescence: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR1D1 protein stained by NR1D1 antibody diluted at 1:400. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574]
Immunohistochemistry-Paraffin: Rev-erb A alpha/NR1D1 Antibody [NBP2-19574] - NR1D1 antibody detects NR1D1 protein at cytosol and nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NR1D1 antibody dilution: 1:500.Applications for Rev-erb A alpha/NR1D1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Rev-erb A alpha/NR1D1
Additional Rev-erb A alpha/NR1D1 Products
Product Documents for Rev-erb A alpha/NR1D1 Antibody
Product Specific Notices for Rev-erb A alpha/NR1D1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.