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Rev-erb beta/NR1D2 Antibody (1G2)

Novus Biologicals, part of Bio-Techne | Catalog # H00009975-M01

Novus Biologicals, part of Bio-Techne
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H00009975-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NR1D2 (AAH45613, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEVNAGGVIAYISSSSSASSHASCHSEGSENSFQSSSSSVPSSPNSSNSDTNGNPKNGDLANIEGILKNDRIDCSMKTSKSSAPGMTKSHSGVTKFSGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKKCLKNENCSIMRMNRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLIEMQSAMKTMMNSQFSGHLQNDTLVEHHEQTALPAQEQLRPKPQLEQENIKSSSPPSSDFAKEEVIGMVTRAHKDTFMYNQEQQENSAESMQPKRGERIRKNMEQYNLNHDHCGNGLSSHFPCSESQQHLNGQFKGRNIMHYPNGHAICIANGHCMNFSNAYTQRVCDRVPIDGFSQNENKNSYLCNTGGRMHLVCPMSKSPYVDPHKSGHEIWEEFSMSFTPAVKEVVEFAKRIPGFRDLSQHDQVNLLKAGTFEVLMVRFASLFDAKERTVTFLSGKKYSVDDLHSMGAGDLLNSMFEFSEKLNALQLSDEEMSLFTAVVLVSADRSGIENVNSVEALQETLIRALRTLIMKNHPNEASIFTKLLLKLPDLRSLNNMHSEELLAFKVHP

Reactivity Notes

Human. Other species not tested.

Specificity

NR1D2 - nuclear receptor subfamily 1, group D, member 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rev-erb beta/NR1D2 Antibody (1G2)

Western Blot: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01]

Western Blot: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01]

Western Blot: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01] - NR1D2 monoclonal antibody (M01), clone 1G2 Analysis of NR1D2 expression in Hela.
Immunocytochemistry/ Immunofluorescence: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01]

Immunocytochemistry/ Immunofluorescence: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01]

Immunocytochemistry/Immunofluorescence: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01] - Analysis of monoclonal antibody to NR1D2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01]

Western Blot: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01]

Western Blot: Rev-erb beta/NR1D2 Antibody (1G2) [H00009975-M01] - Analysis of NR1D2 over-expressed 293 cell line, cotransfected with NR1D2 Validated Chimera RNAi ( Cat # H00009975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D2 monoclonal antibody (M01), clone 1G2 (Cat # H00009975-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for Rev-erb beta/NR1D2 Antibody (1G2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rev-erb beta/NR1D2

The NR1D2 gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Al

Alternate Names

BD73, EAR-1R, NR1D2, Reverb beta

Entrez Gene IDs

9975 (Human)

Gene Symbol

NR1D2

OMIM

602304 (Human)

Additional Rev-erb beta/NR1D2 Products

Product Documents for Rev-erb beta/NR1D2 Antibody (1G2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rev-erb beta/NR1D2 Antibody (1G2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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