RFC2 Antibody (OTI4C11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73858

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RFC2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-234 of human RFC2 (NP_002905) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFC2 Antibody (OTI4C11) - Azide and BSA Free

Western Blot: RFC2 Antibody (OTI4C11)Azide and BSA Free [NBP2-73858]

Western Blot: RFC2 Antibody (OTI4C11)Azide and BSA Free [NBP2-73858]

Western Blot: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2.
Immunohistochemistry: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858]

Immunohistochemistry: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858]

Immunohistochemistry: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858] - Staining of paraffin-embedded Human pancreas tissue using anti-RFC2 mouse monoclonal antibody.
Flow Cytometry: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858]

Flow Cytometry: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858]

Flow Cytometry: RFC2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-73858] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RFC2 antibody, and then analyzed by flow cytometry.
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Applications for RFC2 Antibody (OTI4C11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFC2

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, MGC3665, replication factor C (activator 1) 2, 40kDa, replication factor C 2, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40replication factor C (activator 1) 2 (40kD)

Gene Symbol

RFC2

Additional RFC2 Products

Product Documents for RFC2 Antibody (OTI4C11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RFC2 Antibody (OTI4C11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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