Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all RFC2 Antibodies »

Product Summary for RFC2 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Replication Factor C subunit 2 using the numbering given in entry NP_852136.1 (GeneID 5982).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RFC2 Antibody

Western Blot: RFC2 Antibody [NB100-231]

Western Blot: RFC2 Antibody [NB100-231]

Western Blot: RFC2 Antibody [NB100-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: RFC2 Antibody [NB100-231]

Western Blot: RFC2 Antibody [NB100-231]

Western Blot: RFC2 Antibody [NB100-231] - Whole cell extract (50 ug) from MCF7 and 293T cells (A) or nuclear extract (50 ug) from HeLa cells (B). Antibody used at 0.5 ug/ml (A) and 1 ug/ml (B).

Applications for RFC2 Antibody

Application
Recommended Usage

Western Blot

1:500-1:5000
Application Notes
This antibody can be used for Western Blot. *The investigator should determine the optimal dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFC2

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC2 is the 40 kDa subunit of RFC that is part of the core complex formed with RFC4 and RFC5. RFC2 is also known as replication factor C subunit 2, replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, activator 1 40 kDa subunit, A1 40 kDa subunit, A1, and MGC3665.

Alternate Names

A1, A1 40 kDa subunit, Activator 1 40 kDa subunit, Activator 1 subunit 2, MGC3665, replication factor C (activator 1) 2, 40kDa, replication factor C 2, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40replication factor C (activator 1) 2 (40kD)

Entrez Gene IDs

5982 (Human)

Gene Symbol

RFC2

UniProt

NP_852136.1 (Human)

Additional RFC2 Products

Product Documents for RFC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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