Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A8

Concentration

1 mg/ml

View all RFC4 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RFC4(NP_002907) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFC4 Antibody (OTI1A8)

Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946]

Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946]

Western Blot: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4.
Immunocytochemistry/ Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946]

Immunocytochemistry/ Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946]

Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.
Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946]

Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946]

Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
View 3 more images

Applications for RFC4 Antibody (OTI1A8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RFC4

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, MGC27291, replication factor C (activator 1) 4, 37kDa, Replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37replication factor C (activator 1) 4 (37kD)

Gene Symbol

RFC4

Additional RFC4 Products

Product Documents for RFC4 Antibody (OTI1A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC4 Antibody (OTI1A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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