Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all RFC5 Antibodies »

Product Summary for RFC5 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Replication Factor C subunit 5 using the numbering given in entry NP_031396.1 (GeneID 5985).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RFC5 Antibody

Western Blot: RFC5 Antibody [NB100-235]

Western Blot: RFC5 Antibody [NB100-235]

Western Blot: RFC5 Antibody [NB100-235] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: RFC5 Antibody [NB100-235]

Western Blot: RFC5 Antibody [NB100-235]

Western Blot: RFC5 Antibody [NB100-235]
Western Blot: RFC5 Antibody [NB100-235]

Western Blot: RFC5 Antibody [NB100-235]

Western Blot: RFC5 Antibody [NB100-235] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.

Applications for RFC5 Antibody

Application
Recommended Usage

Western Blot

1:500-1:5000
Application Notes
This antibody can be used for Western Blot. Suggested working dilutions: * Immunoprecipitation ND Immunocytochemistry ND Immunohistochemistry ND *The investigator should determine the optimal dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFC5

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC5 is the 36kDa subunit of RFC that forms a core complex with RFC2 and RFC4 and can interact with the C-terminal region of PCNA. RFC5 is also known as replication factor C 36 kDa subunit RF-C 36 kDa subunit, RFC36, Activator 1 36 kDa subunit, A1 36 kDa subunit, and MGC1155.

Alternate Names

Activator 1 36 kDa subunit, Activator 1 subunit 5,36.5 kD subunit, MGC1155, replication factor C (activator 1) 5, 36.5kDa, RF-C 36 kDa subunit, RFC36replication factor C (activator 1) 5 (36.5kD)

Entrez Gene IDs

5985 (Human)

Gene Symbol

RFC5

UniProt

NP_031396.1 (Human)

Additional RFC5 Products

Product Documents for RFC5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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