RFNG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90946

Novus Biologicals, part of Bio-Techne
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NBP1-90946
NBP1-90946-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RFNG Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AGFCLSRGLALKMSPWASLGSFMSTAEQVRLPDDCTVGYIVEGLLGARLLHSPLFHSHLENLQRLPPDTLLQQVTLSHGGPENPHNVVNVAGGFSLHQDPTRFKSIHCLLYPDTDWCPRQKQGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFNG Antibody

Western Blot: RFNG Antibody [NBP1-90946]

Western Blot: RFNG Antibody [NBP1-90946]

Western Blot: RFNG Antibody [NBP1-90946] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: RFNG Antibody [NBP1-90946]

Immunocytochemistry/ Immunofluorescence: RFNG Antibody [NBP1-90946]

Immunocytochemistry/Immunofluorescence: RFNG Antibody [NBP1-90946] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RFNG Antibody [NBP1-90946]

Immunohistochemistry-Paraffin: RFNG Antibody [NBP1-90946]

Immunohistochemistry-Paraffin: RFNG Antibody [NBP1-90946] - Staining of human kidney shows cytoplasmic, nuclaer and membranous positivity.

Applications for RFNG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFNG

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis

Long Name

Radical Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Alternate Names

beta-1,3-N-acetylglucosaminyltransferase radical fringe, EC 2.4.1.222, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, radical fringe (Drosophila) homolog, radical fringe homolog, radical fringe homolog (Drosophila), RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Gene Symbol

RFNG

Additional RFNG Products

Product Documents for RFNG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFNG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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