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RFX4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48967

Novus Biologicals, part of Bio-Techne
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NBP2-48967
NBP2-48967-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: STTGAMQSYTWSLTYTVTTAAGSPAENSQQLPCMRNTHVPSSSVTHRIPVYPHREEHGYTGSYNYGSYGNQHPHPMQSQYPALPH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFX4 Antibody

Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967]

Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967]

Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967] - Analysis in human testis and endometrium tissues. Corresponding RFX4 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: RFX4 Antibody [NBP2-48967]

Immunocytochemistry/ Immunofluorescence: RFX4 Antibody [NBP2-48967]

Immunocytochemistry/Immunofluorescence: RFX4 Antibody [NBP2-48967] - Staining of human cell line AF22 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967]

Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967]

Immunohistochemistry-Paraffin: RFX4 Antibody [NBP2-48967] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Applications for RFX4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFX4

The RFX4 gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain ahighly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related toregulatory factors X1, X2, X3, and

Alternate Names

NYD-SP10, Regulatory factor X 4, regulatory factor X, 4 (influences HLA class II expression), regulatory factor X4, Testis development protein NYD-SP10, transcription factor NYD-sp10, transcription factor RFX4

Gene Symbol

RFX4

Additional RFX4 Products

Product Documents for RFX4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFX4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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