Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RFX5 Antibodies »

Product Specifications

Immunogen

Rabbits were immunized with a synthetic peptide, which represented a portion of human Regulatory Factor X 5 encoded within exon 5 (LocusLink ID 5993), conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were affinity purified using the peptide immobilized on solid support.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFX5 Antibody

Western Blot: RFX5 Antibody [NB100-195]

Western Blot: RFX5 Antibody [NB100-195]

Western Blot: RFX5 Antibody [NB100-195] - Nuclear extract (50 ug for WB, 1 mg for IP) from HeLa cells. Antibody used at 1 ug/ml for WB and 4 ug for IP.
Western Blot: RFX5 Antibody [NB100-195]

Western Blot: RFX5 Antibody [NB100-195]

Western Blot: RFX5 Antibody [NB100-195] - HeLa Cell Nuclear Extract

Applications for RFX5 Antibody

Application
Recommended Usage

Immunoprecipitation

1 - 4 ug/mg of lysate

Western Blot

1:500-1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFX5

RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.

Alternate Names

DNA-binding protein RFX5, Regulatory factor X 5, regulatory factor X, 5 (influences HLA class II expression)

Entrez Gene IDs

5993 (Human)

Gene Symbol

RFX5

Additional RFX5 Products

Product Documents for RFX5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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