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RG9MTD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88770

Novus Biologicals, part of Bio-Techne
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NBP1-88770
NBP1-88770-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSEILAINQVFDILSTYLETHNWPEALKKGVSSGKGYILRNSV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RG9MTD3 Antibody

Western Blot: RG9MTD3 Antibody [NBP1-88770]

Western Blot: RG9MTD3 Antibody [NBP1-88770]

Western Blot: RG9MTD3 Antibody [NBP1-88770] - Analysis in control (vector only transfected HEK293T lysate) and TRMT10B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RG9MTD3 Antibody [NBP1-88770]

Immunocytochemistry/ Immunofluorescence: RG9MTD3 Antibody [NBP1-88770]

Immunocytochemistry/Immunofluorescence: RG9MTD3 Antibody [NBP1-88770] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770]

Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770]

Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770] - Staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.

Applications for RG9MTD3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RG9MTD3

Probable RNA methyltransferase

Alternate Names

bA3J10.9, EC 2.1.1, EC 2.1.1.-, FLJ31455, RNA (guanine-9-) methyltransferase domain containing 3, RNA (guanine-9-)-methyltransferase domain-containing protein 3, RP11-3J10.9

Gene Symbol

TRMT10B

Additional RG9MTD3 Products

Product Documents for RG9MTD3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RG9MTD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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