RG9MTD3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88770
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSEILAINQVFDILSTYLETHNWPEALKKGVSSGKGYILRNSV
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RG9MTD3 Antibody
Western Blot: RG9MTD3 Antibody [NBP1-88770]
Western Blot: RG9MTD3 Antibody [NBP1-88770] - Analysis in control (vector only transfected HEK293T lysate) and TRMT10B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: RG9MTD3 Antibody [NBP1-88770]
Immunocytochemistry/Immunofluorescence: RG9MTD3 Antibody [NBP1-88770] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770]
Immunohistochemistry-Paraffin: RG9MTD3 Antibody [NBP1-88770] - Staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.Applications for RG9MTD3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RG9MTD3
Alternate Names
bA3J10.9, EC 2.1.1, EC 2.1.1.-, FLJ31455, RNA (guanine-9-) methyltransferase domain containing 3, RNA (guanine-9-)-methyltransferase domain-containing protein 3, RP11-3J10.9
Gene Symbol
TRMT10B
Additional RG9MTD3 Products
Product Documents for RG9MTD3 Antibody
Product Specific Notices for RG9MTD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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