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RGS4 Antibody (HL1522) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25636

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25636

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1522

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS4. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RGS4 Antibody (HL1522) - Azide and BSA Free

RGS4 Antibody (HL1522) - Azide and BSA Free

Western Blot: RGS4 Antibody (HL1522) - Azide and BSA Free [NBP3-25636] -

Western Blot: RGS4 Antibody (HL1522) - Azide and BSA Free [NBP3-25636] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (NBP3-25636) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
RGS4 Antibody (HL1522) - Azide and BSA Free

Western Blot: RGS4 Antibody (HL1522) - Azide and BSA Free [NBP3-25636] -

Western Blot: RGS4 Antibody (HL1522) - Azide and BSA Free [NBP3-25636] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (NBP3-25636) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
RGS4 Antibody (HL1522) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RGS4 Antibody (HL1522) - Azide and BSA Free [NBP3-25636] -

Immunocytochemistry/Immunofluorescence: RGS4 Antibody (HL1522) - Azide and BSA Free [NBP3-25636] - RGS4 antibody [HL1522] detects RGS4 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RGS4 stained by RGS4 antibody [HL1522] (NBP3-25636) diluted at 1:500. Blue: Fluoroshield with DAPI .

Applications for RGS4 Antibody (HL1522) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RGS4

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120 amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat RGS4. This protein negatively regulates signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm.

Long Name

Regulator of G-protein signaling 4

Alternate Names

RGP4

Gene Symbol

RGS4

Additional RGS4 Products

Product Documents for RGS4 Antibody (HL1522) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RGS4 Antibody (HL1522) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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