RGS6 Antibody (12H5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26346
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 12H5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RGS6 Antibody (12H5)
Immunogen
A synthesized peptide derived from Human RGS6 [UniProt P49758]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RGS6 Antibody (12H5)
Immunohistochemistry: RGS6 Antibody (12H5) [NBP3-26346] -
Immunohistochemistry: RGS6 Antibody (12H5) [NBP3-26346] - Image of RGS6 Antibody (12H5) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Immunohistochemistry: RGS6 Antibody (12H5) [NBP3-26346] -
Immunohistochemistry: RGS6 Antibody (12H5) [NBP3-26346] - Image of RGS6 Antibody (12H5) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Immunocytochemistry/Immunofluorescence: RGS6 Antibody (12H5) [NBP3-26346] -
Immunocytochemistry/Immunofluorescence: RGS6 Antibody (12H5) [NBP3-26346] - Staining of PC-3 cell with RGS6 Antibody (12H5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 592-conjugated Goat Anti-Rabbit IgG (H+L).Applications for RGS6 Antibody (12H5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: RGS6
Alternate Names
DKFZp313G1241, FLJ43552, G protein signaling 6 regulator, GAP, GTPase activating protein, H_DJ0283M22.1, H_DJ1108A12.1, MGC142132, regulator of G protein signaling 6, regulator of G protein signalling 6, regulator of G-protein signaling 6, regulator of G-protein signalling 6, S914, WUGSC:H_DJ0283M22.1, WUGSC:H_DJ1108A12.1
Gene Symbol
RGS6
Additional RGS6 Products
Product Documents for RGS6 Antibody (12H5)
Product Specific Notices for RGS6 Antibody (12H5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...