RHAMM/CD168 Antibody (2F2C9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52437
Key Product Details
Species Reactivity
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2F2C9
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human RHAMM/CD168 (AA: 306-497) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
84.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RHAMM/CD168 Antibody (2F2C9) - BSA Free
Western Blot: RHAMM/CD168 Antibody (2F2C9)BSA Free [NBP2-52437]
Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) - BSA Free [NBP2-52437]
Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437AF488 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.Western Blot: RHAMM/CD168 Antibody (2F2C9)BSA Free [NBP2-52437]
Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against human CD168 (AA: 306-497) recombinant protein. (Expected MW is 48.3 kDa)Applications for RHAMM/CD168 Antibody (2F2C9) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RHAMM/CD168
Long Name
Receptor For Hyaluronan-Mediated Motility
Alternate Names
CD168, HMMR, IHABP
Gene Symbol
HMMR
Additional RHAMM/CD168 Products
Product Documents for RHAMM/CD168 Antibody (2F2C9) - BSA Free
Product Specific Notices for RHAMM/CD168 Antibody (2F2C9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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