RHCG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52089

Novus Biologicals, part of Bio-Techne
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NBP1-52089
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RHCG Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ERQNSVYQSD corresponding to internal region according to NP_057405.1.

Epitope

C-ERQNSVYQSD

Reactivity Notes

Expected from sequence similarity: Canine, Porcine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RHCG Antibody

Western Blot: RHCG Antibody [NBP1-52089]

Western Blot: RHCG Antibody [NBP1-52089]

Western Blot: RHCG Antibody [NBP1-52089] - Analysis of RHCG Human Testis lysate (35ug protein in RIPA buffer) using NBP1-52089 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089]

Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089]

Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089] - 5ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089]

Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089]

Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089] - 5ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RHCG Antibody

Application
Recommended Usage

Immunohistochemistry

1-3ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1:100-1:2000
Application Notes
Western blot: Approx 60kDa band observed in Human Testis lysates (calculated MW of 53.2kDa according to NP_057405.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Biol Chem. 2000 Aug 18;275(33):25641-51;PMID: 10852913). Primary incubation was 1 hour
IHC: Paraffin embedded Human Tonsil and Kidney.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RHCG

RHCG functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion

Alternate Names

ammonium transporter Rh type C, CDRC2, chromosome 15 open reading frame 6, PDRC2C15orf6, Rh family type C glycoprotein, Rh family, C glycoprotein, Rh glycoprotein kidney, Rh type C glycoprotein, Rhesus blood group family type C glycoprotein, Rhesus blood group, C glycoprotein, RHGKTumor-related protein DRC2, SLC42A3

Entrez Gene IDs

51458 (Human)

Gene Symbol

RHCG

UniProt

NP_057405.1 (Human)

Additional RHCG Products

Product Documents for RHCG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RHCG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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