Rheb Antibody (1E12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006009-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rheb Antibodies »

Product Specifications

Immunogen

RHEB (AAH16155, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Specificity

Ras homolog enriched in brain

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rheb Antibody (1E12)

Western Blot: Rheb Antibody (1E12) [H00006009-M05]

Western Blot: Rheb Antibody (1E12) [H00006009-M05]

Western Blot: Rheb Antibody (1E12) [H00006009-M05] - Analysis of RHEB expression in transfected 293T cell line by RHEB monoclonal antibody (M05), clone 1E12. Lane 1: RHEB transfected lysatE (20.5 KDa). Lane 2: Non-transfected lysate.
ELISA: Rheb Antibody (1E12) [H00006009-M05]

ELISA: Rheb Antibody (1E12) [H00006009-M05]

ELISA: Rheb Antibody (1E12) [H00006009-M05] - Detection limit for recombinant GST tagged RHEB is approximately 0.1ng/ml as a capture antibody.

Applications for Rheb Antibody (1E12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rheb

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Entrez Gene IDs

6009 (Human)

Gene Symbol

RHEB

OMIM

601293 (Human)

UniProt

AAH16155 (Human)

Additional Rheb Products

Product Documents for Rheb Antibody (1E12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rheb Antibody (1E12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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