Rheb Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31630

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-31630
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Monkey (100%), Mouse (98%), Porcine (99%), Rat (99%), Sheep (99%), Xenopus (91%), Zebrafish (90%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rheb Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rheb. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (91%).

Localization

Cell membrane, Lipid-anchor, Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rheb Antibody

Western Blot: Rheb Antibody [NBP1-31630]

Western Blot: Rheb Antibody [NBP1-31630]

Western Blot: Rheb Antibody [NBP1-31630] - Sample(30 ug whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.

Applications for Rheb Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rheb

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Gene Symbol

RHEB

Additional Rheb Products

Product Documents for Rheb Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rheb Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.