Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Rheb Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb. The immunogen is located within amino acids 40 - 90 of Rheb. Amino Acid Squence: DTAGQDEYSIFPQTY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19, 20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rheb Antibody - BSA Free

Western Blot: Rheb AntibodyBSA Free [NBP1-77214]

Western Blot: Rheb AntibodyBSA Free [NBP1-77214]

Western Blot: Rheb Antibody [NBP1-77214] - Mouse brain cell lysate with Rheb antibody at (A) 1, (B) 2, and (C) 4 ug/mL.
Immunohistochemistry-Paraffin: Rheb Antibody - BSA Free [NBP1-77214]

Immunohistochemistry-Paraffin: Rheb Antibody - BSA Free [NBP1-77214]

Immunohistochemistry-Paraffin: Rheb Antibody [NBP1-77214] - Mouse brain tissue with Rheb antibody at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Rheb Antibody [NBP1-77214] -

Immunocytochemistry/Immunofluorescence: Rheb Antibody [NBP1-77214] - Rheb in Mouse Brain cells with Rheb antibody at 20 ug/mL.
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Applications for Rheb Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rheb

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Long Name

Ras Homolog Enriched In Brain

Alternate Names

Rheb2

Gene Symbol

RHEB

UniProt

AAH16155 (Human)

Additional Rheb Products

Product Documents for Rheb Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rheb Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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