RhoA Antibody (103)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90107

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 103

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RhoA Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human RhoA (Uniprot#: P61586-1; Met1-Leu193).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RhoA Antibody (103)

Western Blot: RhoA Antibody (103) [NBP2-90107]

Western Blot: RhoA Antibody (103) [NBP2-90107]

Western Blot: RhoA Antibody (103) [NBP2-90107] - Anti-RHOA rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody (103) [NBP2-90107]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody (103) [NBP2-90107]

Immunocytochemistry/Immunofluorescence: RhoA Antibody (103) [NBP2-90107] - Immunofluorescence staining of RHOA in Hela cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human RHOA monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for RhoA Antibody (103)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoA

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Alternate Names

h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA

Gene Symbol

RHOA

Additional RhoA Products

Product Documents for RhoA Antibody (103)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoA Antibody (103)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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