RhoA Antibody (7E7H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27177
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 7E7H4
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified recombinant fragment of human RhoA (AA: 1-190) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RhoA Antibody (7E7H4)
Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] -
Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mAb against human RhoA (AA: 1-190) recombinant protein.
Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] -
Western Blot: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis using RhoA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.
Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] -
Flow Cytometry: RhoA Antibody (7E7H4) [NBP3-27177] - Analysis of Jurkat cells using RhoA mouse mAb (green) and negative control (red).
Applications for RhoA Antibody (7E7H4)
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RhoA
Alternate Names
h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, RHO12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA
Gene Symbol
RHOA
Additional RhoA Products
Product Documents for RhoA Antibody (7E7H4)
Product Specific Notices for RhoA Antibody (7E7H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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