RhoB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20154

Novus Biologicals, part of Bio-Techne
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NBP2-20154
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (99%), Chicken (97%), Porcine (100%), Sheep (100%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RhoB Antibodies »

Product Summary for RhoB Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RhoB. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%), Rhesus Monkey (89%).

Localization

Late endosome membrane; Lipid-anchor; Cell membrane; Lipid-anchor; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoB Antibody

Western Blot: RhoB Antibody [NBP2-20154]

Western Blot: RhoB Antibody [NBP2-20154]

Western Blot: RhoB Antibody [NBP2-20154] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RhoB antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: RhoB Antibody [NBP2-20154]

Immunohistochemistry-Paraffin: RhoB Antibody [NBP2-20154]

Immunohistochemistry-Paraffin: RhoB Antibody [NBP2-20154] - detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft.
Western Blot: RhoB Antibody [NBP2-20154]

Western Blot: RhoB Antibody [NBP2-20154]

Western Blot: RhoB Antibody [NBP2-20154] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:500.
View 1 more images

Applications for RhoB Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RhoB

Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.

Alternate Names

ARH6MSTP081, ARHBAplysia RAS-related homolog 6, h6, MST081, oncogene RHO H6, ras homolog gene family, member B, Rho cDNA clone 6, RHOH6RhoB, rho-related GTP-binding protein RhoB

Gene Symbol

RHOB

UniProt

P62745 (Human)

Additional RhoB Products

Product Documents for RhoB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RhoB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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