RhoD Antibody (OTI2F7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01462
![Western Blot: RhoD Antibody (OTI2F7) [NBP2-01462]](https://resources.bio-techne.com/images/products/RhoD-Antibody-2F7-Western-Blot-NBP2-01462-img0002.jpg "Western Blot: RhoD Antibody (OTI2F7) [NBP2-01462]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2F7
Concentration
0.85 mg/ml
Product Summary for RhoD Antibody (OTI2F7)
Immunogen
Full length human recombinant protein of human RHOD(NP_055393) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RhoD Antibody (OTI2F7)
Western Blot: RhoD Antibody (OTI2F7) [NBP2-01462]
Western Blot: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.
Immunocytochemistry/Immunofluorescence: RhoD Antibody (2F7) [NBP2-01462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.
![Flow Cytometry: RhoD Antibody (OTI2F7) [NBP2-01462]](https://resources.bio-techne.com/images/products/RhoD-Antibody-2F7-Flow-Cytometry-NBP2-01462-img0001.jpg "Flow Cytometry: RhoD Antibody (OTI2F7) [NBP2-01462]")
Flow Cytometry: RhoD Antibody (OTI2F7) [NBP2-01462]
Flow Cytometry: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RhoD antibody, and then analyzed by flow cytometry.Applications for RhoD Antibody (OTI2F7)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.85 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RhoD
Alternate Names
ARHDRHOM, ras homolog D, ras homolog gene family, member A, ras homolog gene family, member D, Rho, RhoD, RhoHP1, rho-related GTP-binding protein RhoD, Rho-related protein HP1
Entrez Gene IDs
29984 (Human)
Gene Symbol
RHOD
Additional RhoD Products
Product Documents for RhoD Antibody (OTI2F7)
Product Specific Notices for RhoD Antibody (OTI2F7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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