Rhodopsin Antibody (A531)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25159

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Equine

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A531

Concentration

1 mg/ml

View all Rhodopsin Antibodies »

Product Specifications

Immunogen

Whole purified bovine Rhodopsin [UniProt# P02699]

Epitope

epitope resides in the N-terminal 32 amino acids

Localization

Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rhodopsin Antibody (A531)

Western Blot: Rhodopsin Antibody (A531) [NBP2-25159]

Western Blot: Rhodopsin Antibody (A531) [NBP2-25159]

Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.
Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159]

Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159]

Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159] - Pig retinal section stained with NBP2-25159 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).

Applications for Rhodopsin Antibody (A531)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:5000
Application Notes
This Rhodopsin (A531) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~35 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rhodopsin

Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the Rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, Rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark Rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the Rhodopsin making it catalytically active. Activated Rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha and a beta gamma subunit. The alpha subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolysescyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain. Rhodopsin activity is shut off by phosphorylation under the influence of Rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents Rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways.

Long Name

Rhodopsin

Alternate Names

OPN2, Opsin-2, RHO

Entrez Gene IDs

6010 (Human)

Gene Symbol

RHO

OMIM

180380 (Human)

UniProt

P08100 (Human)

Additional Rhodopsin Products

Product Documents for Rhodopsin Antibody (A531)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rhodopsin Antibody (A531)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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