RhoF Antibody (1D4B6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52553

Novus Biologicals, part of Bio-Techne
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NBP2-52553
NBP2-52553-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1D4B6

Format

BSA Free

Concentration

1 mg/ml

View all RhoF Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RHOF (AA: 1-84) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoF Antibody (1D4B6) - BSA Free

Western Blot: RhoF Antibody (1D4B6)BSA Free [NBP2-52553]

Western Blot: RhoF Antibody (1D4B6)BSA Free [NBP2-52553]

Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against human RHOF (AA: 1-84) recombinant protein. (Expected MW is 34.9 kDa)
Flow Cytometry: RhoF Antibody (1D4B6) - BSA Free [NBP2-52553]

Flow Cytometry: RhoF Antibody (1D4B6) - BSA Free [NBP2-52553]

Flow Cytometry: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).
Western Blot: RhoF Antibody (1D4B6)BSA Free [NBP2-52553]

Western Blot: RhoF Antibody (1D4B6)BSA Free [NBP2-52553]

Western Blot: RhoF Antibody (1D4B6) [NBP2-52553] - Analysis using RHOF mAb against HEK293 (1) and RHOF (AA: 1-84)-hIgGFc transfected HEK293 (2) cell lysate.
View 1 more images

Applications for RhoF Antibody (1D4B6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoF

Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.; Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Cytoplasm > cytoskeleton.

Alternate Names

FLJ20247, ras homolog gene family, member F (in filopodia), Rho family GTPase Rif, Rho in filopodia, rho-related GTP-binding protein RhoF, RIFARHF

Gene Symbol

RHOF

Additional RhoF Products

Product Documents for RhoF Antibody (1D4B6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoF Antibody (1D4B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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