RhoF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98649

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RhoF Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human RhoF fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RhoF Antibody

Western Blot: RhoF Antibody [NBP2-98649]

Western Blot: RhoF Antibody [NBP2-98649]

Western Blot: RhoF Antibody [NBP2-98649] - Anti-RhoF rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa
Immunoprecipitation: RhoF Antibody [NBP2-98649]

Immunoprecipitation: RhoF Antibody [NBP2-98649]

Immunoprecipitation: RhoF Antibody [NBP2-98649] - RhoF was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-RhoF rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RhoF rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa

Applications for RhoF Antibody

Application
Recommended Usage

Immunoprecipitation

5-10uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoF

Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.; Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Cytoplasm > cytoskeleton.

Alternate Names

FLJ20247, ras homolog gene family, member F (in filopodia), Rho family GTPase Rif, Rho in filopodia, rho-related GTP-binding protein RhoF, RIFARHF

Gene Symbol

RHOF

Additional RhoF Products

Product Documents for RhoF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RhoF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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