RhoGAP Antibody (3L9I3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16114

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16114-100ul
NBP3-16114-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3L9I3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RhoGAP Antibodies »

Product Summary for RhoGAP Antibody (3L9I3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1150-1300 of human RhoGAP (Q13017). PSKYKYKSKTLFSKAKSYYRRTHSDASDDEAFTTSKTKRKGRHRGSEEDPLLSPVETWKGGIDNPAITSDQELDDKKMKKKTHKVKEDKKQKKKTKNFNPPTRRNWESNYFGMPLQDLVTAEKPIPLFVEKCVEFIEDTGLCTEGLYRVSG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RhoGAP Antibody (3L9I3)

Western Blot: RhoGAP Antibody (3L9I3) [NBP3-16114]

Western Blot: RhoGAP Antibody (3L9I3) [NBP3-16114]

Western Blot: RhoGAP Antibody (3L9I3) [NBP3-16114] - Western blot analysis of extracts of various cell lines, using RhoGAP Rabbit mAb (NBP3-16114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: RhoGAP Antibody (3L9I3) [NBP3-16114]

Immunoprecipitation: RhoGAP Antibody (3L9I3) [NBP3-16114]

Immunoprecipitation: RhoGAP Antibody (3L9I3) [NBP3-16114] - Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug RhoGAP antibody (NBP3-16114). Western blot was performed from the immunoprecipitate using RhoGAP antibody (NBP3-16114) at a dilition of 1:500.

Applications for RhoGAP Antibody (3L9I3)

Application
Recommended Usage

Immunoprecipitation

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoGAP

The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3).

Alternate Names

growth factor independent 2, p100 RasGAP-associated p105 protein, p105 RhoGAP, p190-BGFI2, p190BRhoGAP, Rho GTPase activating protein 5, rho GTPase-activating protein 5, RhoGAP5, Rho-type GTPase-activating protein 5

Gene Symbol

ARHGAP5

Additional RhoGAP Products

Product Documents for RhoGAP Antibody (3L9I3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RhoGAP Antibody (3L9I3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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